MonoStereo Visualization System is designed to have the stereo visualization from a monoscopic image during the Minimally Invasive Surgery (MIS). It provides real-time display and adjustable 3D effects to minimally invasive surgery. Since 2D rigid and flexible endoscope are both emerging tools for MIS and interventions, unfortunately, depth perception is lost in 2D endoscopic capturing and display. It makes surgical and interventional tasks harder and difficult to recognize the tissue lesions. The system works well with all HD 2D endoscope systems, not only keep 2D features as room-in, room-out, and rotation but also see 3D effects with depth perception and adjustable 3D disparity.